Imag MiR Not Pro

Download pose: MicroRNAs (miRNA) are short noncoding RNAs that can play critical roles in diverse biolorocesses. They are implicated in tumorigenesis and function both as tumor suppressors and as enes. The clinical significance of miRNA expression profiles in malignant gliomas remains unclear. erimental Design: In this study, we examined the expression levels of 365 mature human miRNAs malignant gliomas, including 8 glioblastomas and 4 anaplastic astrocytomas, using TaqMan realuantitative PCR arrays. A validation study was done to corroborate a subset of the results, including sion levels of miR-196a, -196b, -21, and -15b, by analyzing 92 malignant gliomas by conventional me PCR. We modeled the relationship between the expression levels of these miRNAs and the al rate of 39 glioblastoma patients by Kaplan-Meier method and multivariate analysis. ults: Expression profiles in glioblastomas and anaplastic astrocytomas suggested that 16 miRNAs andidate markers associated with the malignant progression of gliomas. Among them, miR-196a d the most significant difference (P = 0.0038), with miR-196b also having a high significance .0371). Both miRNAs showed increased expression levels in glioblastomas relative to both anaastrocytomas and normal brains in the validation study. Furthermore, patients with high miRxpression levels showed significantly poorer survival by the Kaplan-Meier method (P = 0.0073). ariate analysis showed that miR-196 expression levels were an independent predictor of overall al in all 39 glioblastoma patients (P = 0.021; hazard ratio, 2.81). surviv Conclusions: Our results suggest that miR-196 may play a role in the malignant progression of gliomas and may be a prognostic predictor in glioblastomas. Clin Cancer Res; 16(16); 4289–97. ©2010 AACR.